Product Description

Dok-2 (phospho-Y299) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4786-50, BS4786-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9, 22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of “docking” proteins which contain mμltiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimμlation by a number of growth factors, including PDGF, VEGF, insμlin and IGF. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), DOK2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Dok-2 around the phosphorylation site of Tyrosine 299.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Dok-2 (Y299) polyclonal antibody detects endogenous levels of Dok-2 protein when phosphorylated at Tyr299.

Molecular Weight: ~ 56 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O60496

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only