Product Description

Dok-2 (R293) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2637-50, BS2637-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60496

Host: Rabbit

Reactivity: Human, Mouse

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of “docking” proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Dok-2 (R293) pAb in paraffin-embedded human breast carcinoma tissue.

Bioworld Molecular Weight: ~ 56 kDa

Specificity: Dok-2 (R293) polyclonal antibody detects endogenous levels of Dok-2 protein.

Alternative Name: Docking protein 2; Downstream of tyrosine kinase 2; p56(dok-2); DOK2

Immunogen: Synthetic peptide, corresponding to amino acids 271-320 of Human Dok-2.

Conjugate: Unconjugated

Modification: Unmodified