Doublecortin Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90426-50, BS90426-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: O43602(Human) O88809(Mouse) Q9ESI7(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, FC
All Applications: WB:1:1,000-1:5,000
FC:1:50-1:100
Background: Lissencephaly (smooth brain) is an abnormality of brain development characterized by incomplete neuronal migration and a smooth cerebral surface, resulting in severe mental retardation. Genetic analysis identified two proteins that are mutated in some cases of lissencephaly, designated lissencephaly-1 protein (LIS1) and doublecortin. LIS1 shows sequence homology to β-subunits of heterotrimeric G proteins. Doublecortin contains a consensus Abl phosphorylation site, and it has some sequence homology to a predicted kinase protein. Both proteins are highly expressed in developing brain, suggesting that they may be involved in a signal transduction pathway that is crucial to brain development.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Doublecortin polyclonal antibody detects endogenous levels of Doublecortin protein.
Bioworld Molecular Weight: 41 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Doublecortin on human brain lysates using anti-Doublecortin antibody at 1/1,000 dilution., Flow cytometric analysis of HepG2 cells with Doublecortin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Alternative Name: Lis-X, DCX, DBCN, Dbct, DC, DCX, DCX, Doublecortex, Doublin, FLJ51296, Lis X, Lis-X, Lissencephalin X, Lissencephalin-X, Lissencephaly X linked, Lissencephaly X linked doublecortin, LISX, Neuronal migration protein doublecortin, OTTHUMP00000023859, OTTHUMP00000023860, OTTHUMP00000216315, OTTHUMP00000216316, SCLH, XLIS,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified