DPF2 (E174) Polyclonal Antibody-BS2668

DPF2 (E174) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2668-50, BS2668-100

Sizes: 50µl, 100µl

Swiss-Prot: Q92785

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: DPF2 (D4, zinc and double PHD fingers family 2), also known as REQ (Requiem), UBID4 or ubi-d4, is a 391 amino acid protein that is a member of the D4 domain family. DPF2, a ubiquitously expressed protein, localizes to the nucleus and contains one C2H2- and two PHD-type zinc finger motifs. DPF2 may function as a transcription factor that is necessary for apoptosis and may also play a role in the development and maturation of lymphoid cells. It is thought that, during apoptosis, DPF2 activity is inhibited by LRF (Leukemia/lymphoma-related factor), which is upregulated by integrin. This suggests that DPF2 may be a potential target for future cancer therapies that induce apoptosis in leukemia cells. Alternative splicing of this gene generates multiple isoforms lacking certain domain.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: DPF2 (E174) polyclonal antibody detects endogenous levels of DPF2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DPF2 (E174) polyclonal antibody at 1:1000 dilution Lane 1: THP-1 whole cell lysate (37ug)
Lane 2: MCF-7 whole cell lysate (48ug) Lane 3: RAW264.7 whole cell lysate (57ug) Lane 4: NIH3T3 whole cell lysate (48ug) Lane 5: H9C2 whole cell lysate (37ug) , Immunohistochemistry (IHC) analyzes of DPF2 (E174) pAb in paraffin-embedded human breast cancer tissue.

Bioworld Molecular Weight: ~ 44 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; D4; D4 zinc and double PHD fingers family 2; double PHD fingers family 2; DPF 2; DPF2; MGC10180; Protein requiem; REQ; REQU; Requiem; Requiem apoptosis response zinc finger; UBI D4; ubi-d4; UBID 4; UBID4; zinc and double PHD fingers family 2; Zinc finger protein ubi d4;

Immunogen: Synthetic peptide, corresponding to amino acids 140-188 of Human DPF2.

Conjugate: Unconjugated

Modification: Unmodified