DROSHA Polyclonal Antibody
Catalogue Numbers: BS61337-50, BS61337-100
Sizes: 50µl, 100µl
Alternative Name: Ribonuclease 3 (EC:3.1.26.3); Protein Drosha; Ribonuclease III; RNase III; p241; DROSHA; RN3; RNASE3L; RNASEN
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9NRR4
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: DROSHA polyclonal antibody detects endogenous levels of DROSHA protein.
Bioworld Molecular Weight: ~ 159 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of DROSHA polyclonal antibody at 1:500 dilution
Lane 1: HEPG2 whole cell lysate
Lane 2: The Brian tissue lysate of Rat
Lane 3: The Brian tissue lysate of Mouse
Immunogen: A synthetic peptide corresponding to residues in Human DROSHA.
Conjugate: Unconjugated
Modification: Unmodified