DUSP14 Polyclonal Antibody-BS60040

DUSP14 Polyclonal Antibody

Catalogue Numbers: BS60040-50, BS60040-100

Sizes: 50µl, 100µl

Alternative Name: Dual specificity protein phosphatase 14; MKP-1-like protein tyrosine phosphatase; MKP-L; Mitogen-activated protein kinase phosphatase 6; MAP kinase phosphatase 6; MKP-6; MKP6

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95147

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The deduced 198 amino acid MAP kinase phosphatase 6 (MKP-6), also designated MAP6 and dual-specificity phosphatase 14 (DUSP14), is homologous to other MKP family proteins in that it has a conserved, centrally located, catalytic core, but differs from traditional MKP proteins because it contains unique N- and C-terminal regions. Binding and deletion analyses have established that the interaction between the cytoplasmic tail of CD28 (a T cell antigen) and MKP-6 occurs at Tyr 200 of CD28 and is specific for both MKP-6 and CD28; however, Tyr 200 can be mutated to Phe 200 without a loss of binding ability. Functional analysis indicates that MKP-6 dephosphorylates ERK, JNK and p38 while acting as a negative regulator of CD28 signaling. MKP-6 is expressed ubiquitously, although expression is stronger in certain cell types and tissues than in others.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DUSP14 polyclonal antibody detects endogenous levels of DUSP14 protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DUSP14 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Rat testis tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human DUSP14.

Conjugate: Unconjugated

Modification: Unmodified