Product Description

DUSP3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65945-50, BS65945-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P51452

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Sheep,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DUSP3 Polyclonal Antibody detects endogenous levels of DUSP3 protein.

Bioworld Molecular Weight: 20kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes;Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DUSP3) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: Dual specificity phosphatase 3; Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUS3_HUMAN; DUSP3; Serine/threonine specific protein phosphatase; Vaccinia H1-related phosphatase; Vaccinia virus phosphatase VH1 related; VHR.

Immunogen: KLH conjugated synthetic peptide derived from human VHR/DUSP3:41-140/185

Conjugate: Unconjugated

Modification: Unmodified