Dynamin 2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90433-50, BS90433-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P50570(Human) P39054(Mouse) P39052(Rat)
Host: Rabbit
Reactivity: Human
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:500-1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Members of the dynamin family, including Dynamin I and Dynamin II, are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin I is localized to the central nervous system, while Dynamin II exhibits ubiquitous distribution with highest expression found in testis. Both dynamin proteins contain SH3 and proline-rich domains that mediate interactions between the dynamins and effectors of their GTPase activity. The interactions with these effectors, which include microtubules, acidic phospholipids and SH3 domain-containing proteins, are required for rapid endocytosis. Dynamin I appears to be recruited to clathrin coated pits by SH3 domain interaction with amphiphysin, a protein highly expressed in brain.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: Dynamin 2 polyclonal antibody detects endogenous levels of Dynamin 2 protein.
Bioworld Molecular Weight: 98 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Dynamin 2 on different cell lysate using anti-Dynamin 2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: Mouse thymus
Lane 3: Hela, ICC staining Dynamin 2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: DNM2, DYN2, CMT2M, CMTDI1, CMTDIB, DI CMTB, Dnm2, DYN II, DYN2, DYN2, Dynamin II, Dynamin-2, Dynamin2, DynaminII, DYNII,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified