Product Description

E2F1 (phospho-T433) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4229-50, BS4229-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The E2F transcription factors are essential for regμlation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control. E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regμlated by a PI3-kinase-like kinase family such as the ATM/ATR kinases.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: IHC

Alternate Names: Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1, RBBP3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human E2F-1 around the phosphorylation site of Threonine 433.

Dilution: IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-E2F1 (T433) polyclonal antibody detects endogenous levels of E2F1 protein only when phosphorylated at Thr433

Molecular Weight: ~ 47 kDa

Pathway: Cell Cycle Control G1 S Checkpoint,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q01094

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only