E2F2 Polyclonal Antibody-BS90439

E2F2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90439-50, BS90439-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14209(Human) P56931(Mouse) EntrezGene:684111 (Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: E2F2 polyclonal antibody detects endogenous levels of E2F2 protein.

Bioworld Molecular Weight: 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of E2F2 on different lysates using anti-E2F2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: 293T
Lane 3: HepG2, Flow cytometric analysis of K562 cells with E2F2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: E2F-2, E2F2, dE2F2, E2F transcription factor 2, E2F-2, E2F2, E2F2, Transcription factor E2F2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified