E4BP4 (E65) Polyclonal Antibody-BS2076

E4BP4 (E65) Polyclonal Antibody

Catalogue Numbers: BS2076-50, BS2076-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16649

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: E4BP4, also known as NFIL3, functions as a transcriptional repressor and is a member of the basic leucine zipper (bZIP) transcription factor family. E4BP4 binds with high specificity to the E4 ATF, which is a DNA sequence traditionally targeted by the ATF/CREB family of transcription factors. A 65 amino acid segment located in the carboxy-terminus of E4BP4 interacts specifically with the TBP binding protein Dr1. In the suprachiasmatic nucleus, circadian center and liver, E4BP4 competes with PAR proteins for DNA binding via a reciprocating mechanism. The phase expression of E4BP4 correlates with the circadian cycle and represses transcription of genes otherwise activated by PAR transcription regulators. E4BP4 also plays an important role in an IL-3-mediated signaling pathway that is responsible for the survival of B cell progenitors. The gene encoding human E4BP4 maps to chromosome 9q22.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: E4BP4 (E65) polyclonal antibody detects endogenous levels of E4BP4protein.

Extra Notes: Western blot (WB) analysis of E4BP4 (E65) pAb at 1:500 dilution
Lane1:A549 whole cell lysate(40ug)
Lane2:SKOVCAR3 whole cell lysate(40ug)
Lane3:A2780 whole cell lysate(40ug)
Lane4:H9C2 whole cell lysate(40ug)
Lane5:BV2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: E4 promoter binding protein 4; E4 promoter-binding protein 4; E4BP 4; E4BP4; E4BP4 protein; IL3BP 1; IL3BP1; Interleukin 3 binding protein 1; Interleukin 3 promoter transcriptional activator; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NFIL 3; NFIL 3A; NFIL3; NFIL3; NFIL3A; Nuclear factor interleukin 3 regulated; Nuclear factor interleukin-3-regulated protein; Transcriptional activator NF IL3A; Transcriptional activator NF-IL3A

Immunogen: Synthetic peptide, corresponding to amino acids 32-86 of Human E4BP4.

Conjugate: Unconjugated

Modification: Unmodified