Product Description

EAAT2 Polyclonal Antibody

Catalogue Numbers: BS67505-50, BS67505-100

Sizes: 50µl, 100µl

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P43004

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. During neurotransmission, glutamate is released from vesicles of the pre-synaptic cell, and glutamate receptors (e.g. NMDA Receptor, AMPA Receptor) bind glutamate for activation at the opposing post-synaptic cell. Excitatory amino acid transporters (EAATs) regulate and maintain extracellular glutamate concentrations below excitotoxic levels. In addition, glutamate transporters may limit the duration of synaptic excitation by an electrogenic process in which the transmitter is cotransported with three sodium ions and one proton, followed by countertransport of a potassium ion. Five EAATs (EAAT1-5) are characterized: EAAT2 (GLT-1) is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development. Homozygous EAAT2 knockout mice have spontaneous, lethal seizures and an increased predisposition to acute cortical injury. PKC phosphorylates Ser113 of EAAT2 and coincides with glutamate transport .

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of EAAT2 protein.

Extra Notes: Western blot analysis of EAAT2 expression in rat brain (A), mouse brain (B) whole cell lysates.

Bioworld Molecular Weight: ~ 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: EAAT2; GLT1; Excitatory amino acid transporter 2; Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EAAT2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified