EDEM1 Polyclonal Antibody
Catalogue Numbers: BS60705-50, BS60705-100
Sizes: 50µl, 100µl
Alternative Name: ER degradation-enhancing alpha-mannosidase-like protein 1; EDEM1; EDEM; KIAA0212
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q92611
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Proteins expressed in the endoplasmic reticulum (ER) are subjected to a tight quality control. Terminally misfolded proteins in the endoplasmic reticulum (ER) are retrotranslocated to the cytoplasm and degraded by proteasomes through a mechanism known as ER-associated degradation (ERAD). EDEM (ER degradation-enhancing alpha-mannosidase-like) protein is a type II membrane protein that localizes to the ER and is directly involved in ERAD. EDEM targets misfolded glycoproteins for degradation in an N-glycan-dependent manner and extracts misfolded glycoproteins from the calnexin cycle. The human EDEM gene maps to chromosome 3p.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: EDEM1 polyclonal antibody detects endogenous levels of EDEM1 protein.
Bioworld Molecular Weight: ~ 74 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of EDEM1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human EDEM1
Conjugate: Unconjugated
Modification: Unmodified