Product Description

EDN1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6036-50, BS6036-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05305

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB

All Applications: WB 1:500 - 1:2000

Background: The human endothelins represent a gene family comprised of endothelin-1, endothelin-2, and endothelin-3, also known as ET-1, ET-2, and ET-3. Endothelins can affect the central nervous system and neuronal excitability, and they elicit potent vasoconstrictor action. The two receptor subtypes responsible for inducing vasoconstriction and vasodilation, ETA and ETB, have different receptor affinities for ET-1, ET-2, and ET-3. Of the three isopeptides, ET-2 has the most potent vasoconstrictor activity (1). Biologically active ETs are proteolytically generated from a larger precursor, the big-endothelin, by action of the endothelin-converting enzyme (ECE) family. ET-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EDN1 polyclonal antibody detects endogenous levels of EDN1 protein.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EDN1 pAb at 1:500 dilution
Lane A: HEK293T whole cell lysate(40ug)
Lane B: LOVO whole cell lysate(40ug)
Lane C: H1792 whole cell lysate(40ug)
Lane D: The Testis tissue lysate of Mouse(40ug)

Alternative Name: Big endothelin 1; Big endothelin-1; EDN 1; EDN1; EDN1 protein; EDN1; Endothelin1; ET 1; ET-1; ET1; OTTHUMP00000039245; PPET 1; PPET1; Preproendothelin 1; Preproendothelin-1; Preproendothelin1

Immunogen: Synthetic peptide, corresponding to Human EDN1.

Conjugate: Unconjugated

Modification: Unmodified