eEF1A1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90445-50, BS90445-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P68104(Human) P10126(Mouse) P62630(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IF, IHC FC IP,
All Applications: WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The elongation factor-1 complex is composed of two subunits, EF-1 α1 (elongation factor 1-alpha 1) and EF-1 α2 (elongation factor 1-alpha 2), and is responsible for the delivery of aminoacyl tRNAs to the ribosome. EF-1 α1 is expressed predominately in brain, placenta, lung, liver, kidney and pancreas, while EF-1 α2 is highly expressed in heart, brain and skeletal muscle. Both EF-1 α1 and α2 localize to the nucleus and belong to the GTP-binding elongation factor family. The gene encoding EF-1 α2, which maps to human chromosome 20q13.3, may play a role in the development of ovarian cancer, while the EF-1 α1 gene, mapping to chromosome 6Q14.1, is commonly present as an autoantigen in patients with Felty syndrome. Felty syndrome is a disorder characterized by rheumatoid arthritis, a swollen spleen, decreased white blood cell count, and increased susceptibility to infection.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: eEF1A1 polyclonal antibody detects endogenous levels of eEF1A1 protein.
Bioworld Molecular Weight: 50 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution.
Positive control:
Lane 1: Rat brain tissue
Lane 2: Mouse skeletal muscle
Lane 3: Mouse cerebellum
Lane 4: Rat skin
Lane 5: Daudi, ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: EF-1-alpha-1, EF-Tu, eEF1A-1, EEF1A1, EEF1A, EF1A, LENG7, CCS 3, CCS3, Cervical cancer suppressor 3, chunp6927, CTCL tumor antigen, EE1A1, EEF 1, EEF1A, eEF1A-1, EEF1A1, EF-1-alpha-1, EF-Tu, EF1A, EF1a like protein, EF1A1, Elongation factor 1 alpha subunit, Elongation factor 1-alpha 1, Elongation factor Tu, Eukaryotic elongation factor 1 A-1, Eukaryotic translation elongation factor 1 alpha 1, Eukaryotic translation elongation factor 1 alpha 1 like 14, Glucocorticoid receptor AF 1 specific elongation factor, GRAF 1EF, HNGC:16303, ik:tdsubc_2a3, ik:tdsubc_2b3, LENG7, Leukocyte receptor cluster (LRC) member 7, Leukocyte receptor cluster member 7, Prostate tumor inducing protein 1, PTI1, tdsubc_2a3, Translation elongation factor 1 alpha 1 like 14, wu:fa91c07, wu:fa94b03, wu:fi13b09, xx:tdsubc_2a3, xx:tdsubc_2b3,
Immunogen: Recombinant protein
Conjugate: Unconjugated
Modification: Unmodified