Product Description

EEF2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB0020-50, MB0020-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P13639

Host: Mouse

Reactivity: Human

Applications: WB

All Applications: WB: 1:1000

Background: Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.

Purification and Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of EEF2 and does not cross-react with related proteins

Bioworld Molecular Weight: Predicted band size:95KDa
Observed band size:95KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western BlotDetection of EEF2 in HL-60 cell lysates using EEF2 antibody (1:1000 diluted).

Alternative Name: Elongation factor 2; EF-2; EEF2; EF2

Immunogen: Recombinant full length Human EEF2.

Conjugate: Unconjugated

Modification: Unmodified