Product Description

EEF2 (phospho-T56) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4059-50, BS4059-100

Swiss-Prot: P13639

Host: Rabbit

EEF2 (phospho-T56) Polyclonal Antibody-BS4059

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis. EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated. EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-EEF2 (T56) polyclonal antibody detects endogenous levels of EF-2 protein when phosphorylated at Thr56.

Bioworld Molecular Weight: ~ 95 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-EEF2 (T56) pAb at 1:500 dilution
Lane 1: K562 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (10ug)
Lane 3: The Liver tissue lysate of Rat (40ug)
Lane 4: The Liver tissue lysate of Mouse (40ug), Immunohistochemistry (IHC) analyzes of p-EEF2 (T56) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: Elongation factor 2; EF-2; EEF2; EF2

Immunogen: Synthetic peptide, corresponding to amino acids 20-70 of Human eEF2.

Modification: Phosphorylation

Conjugate: Unconjugated