Product Description

eEF2K (phospho-S366) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4060-50, BS4060-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: eEF2K is 105 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38γ, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insμlin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this resμlts in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC, IP

Alternate Names: Elongation factor 2 kinase, eEF-2 kinase, eEF-2K, Calcium/calmodμlin-dependent eukaryotic elongation factor 2 kinase, EEF2K

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human eEF2K around the phosphorylation site of Serine 366.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200
IP: 1:10~1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-eEF2K (S366) polyclonal antibody detects endogenous levels of eEF2K protein when phosphorylated at Ser366.

Molecular Weight: ~ 105 kDa

Pathway: Signaling Pathways Activating p38 MAP Kinase,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O00418

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only