Product Description

EF-Tu (L338) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS9146-50, BS9146-100

Sizes: 50µl, 100µl

Swiss-Prot: P49411

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: EF-Ts, also known as TSFM (Ts translation elongation factor, mitochondrial) or COXPD3, is a 325 amino acid protein that is one of 13 mitochondrial-encoded proteins that work together during the elongation phase of protein biosynthesis on the ribosome. Expressed ubiquitously with highest levels present in liver, kidney and skeletal muscle, EF-Ts associates with EF-Tu, a multidomain GTPase with essential functions in translation, and, via this interaction, facilitates the exchange of GDP for GTP, thereby inducing protein elongation. Mutations in the gene encoding EF-Ts are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3), a condition characterized by defects in the mitochondrial oxidative phosphorylation system and often characterized by severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Multiple isoforms of EF-Ts exist due to alternative splicing events.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: EF-Tu (L338) polyclonal antibody detects endogenous levels of EF-Tu protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EF-Tu (L338) pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate (40ug)
Lane 2: A549 whole cell lysate (40ug)
Lane 3: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of EF-Tu (L338) pAb in paraffin-embedded human lung cancer tissue.

Bioworld Molecular Weight: ~ 43 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Elongation factor Tu, mitochondrial; EFTu; P43; TUFM

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human EF-Tu.

Conjugate: Unconjugated

Modification: Unmodified