Eg5 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90446-50, BS90446-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P52732(Human) Q6P9P6(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, FC
All Applications: ICC:1:50
IHC:1:50-1:200
FC:1:50-1:100
Background: Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.
Purification and Purity: Protein affinity purified.
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: Eg5 polyclonal antibody detects endogenous levels of Eg5 protein.
Bioworld Molecular Weight: 119 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Eg5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Eg5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: TR-interacting protein 5, TRIP-5, KIF11, EG5, KNSL1, TRIP5, EG5, HKSP, KIF11, KIF11, Kinesin family member 11, Kinesin like protein 1, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, KNSL1, MCLMR, Thyroid receptor-interacting protein 5, TR-interacting protein 5, TRIP-5, TRIP5,
Immunogen: Recombinant protein within human Eg5 aa 100-300.
Conjugate: Unconjugated
Modification: Unmodified