EGFR Mouse mAb - EM1044

EGFR Mouse mAb

Sizes: 50μL, 100μL

Catalogue Numbers: EM1044-50, EM1044-100

Citations, Manuals and MSDS Available upon request.

Background: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascadesβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

Alternate Name: Epidermal Growth Factor Receptor ; ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral (v-Erb-B)oncogene Homolog, Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Source: Mouse

Applications: WB, IHC, IF

Dilution: WB 1:1, 000 IF 1; 100-200  IHC 1:200-500

Reactivity: Human

Immunogen: Synthetic Peptide

Storage and Stability: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Clonality: Monoclonal

Isotype: IgG1

Concentration: 1mg/mL

Observed Band (KD): 170kDa

Human Gene ID: 1956

Human SWISS Prot NO: P00533

Subcellular Location: Secreted and Cell membrane. Endoplasmic reticulum membrane

Research Use Only