Product Description

EGR1 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS80415-50, BS80415-100

Swiss-Prot: P18146

Host: Rabbit

EGR1 Polyclonal Antibody-BS80415

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:200 - 1:1000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 75KDa/100KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using EGR1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s., Immunofluorescence analysis of U2OS cells using EGR1 Rabbit pAb at dilution of 1:250. Blue: DAPI for nuclear staining.

Alternative Name: EGR1; AT225; G0S30; KROX-24; NGFI-A; TIS8; ZIF-268; ZNF225

Immunogen: Recombinant fusion protein of human EGR1(NP_001955.1).

Modification: Unmodified

Conjugate: Unconjugated