eIF-2a (Phospho-S51) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94029-50, BS94029-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9BY44(Human) Q8BJW6(Mouse) EntrezGene:502531(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex. eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B. Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: eIF-2a (Phospho-S51) polyclonal antibody detects endogenous levels of eIF-2a protein only when phosphorylated at S51.
Bioworld Molecular Weight: 36 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-eIF-2a(S51) on different lysates using anti-Phospho-eIF-2a(S51) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HUVEC
Lane 3: PC12, ICC staining Phospho-eIF-2a(S51) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: EIF2A, CDA02, MSTP004, MSTP089, EIF 2 alpha, EIF 2, EIF 2A, EIF 2alpha, eIF-2-alpha, eIF-2A, EIF-2alpha, EIF2 alpha, EIF2, EIF2A, EIF2S1, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, Eukaryotic translation initiation factor 2 subunit 1 alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, IF2A,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a.
Conjugate: Unconjugated
Modification: Phosphorylation