Product Description

EIF2B3 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB67223-50, MB67223-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q9NR50

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC, FC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50), FC (1/10 - 1/50)

Background: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of EIF2B3 protein.

Bioworld Molecular Weight: ~ 56 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of EIF2B3 expression in HL60 (A), MCF7 (B), U2OS (C), K562 (D) whole cell lysates., Immunofluorescent analysis of EIF2B3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Alternative Name: Translation initiation factor eIF-2B subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EIF2B3. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified