EIF2B4 Polyclonal Antibody-BS62501

EIF2B4 Polyclonal Antibody

Catalogue Numbers: BS62501-50, BS62501-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UI10

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2B exists as a five subunit complex composed of eIF2Bα, eIF2Bβ, eIF2Bγ, eIF2Bδ and eIF2Bε. The eIF2B complex catalyzes the exchange of GDP for GTP on the eIF2 complex, following the interaction of eIF2/GTP with the 40S ribosomal subunit. Guanine nucleotide exchange factor (GEF) activity was exhibited by the eIF2Bε subunit alone, but it was greater in the presence of all five eIF2B subunits. Phosphorylation of eIF2 inhibits GEF activity of eIF2B, an inhibition that requires the eIF2Bα subunit.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EIF2B4 polyclonal antibody detects endogenous levels of EIF2B4 protein.

Extra Notes: Western blot (WB) analysis of EIF2B4 polyclonal antibody at 1:500 dilution
LaneA:Hela whole cell lysate
LaneB:MCF-7 whole cell lysate
LaneC:PC12 whole cell lysate
LaneD:CT26 whole cell lysate

Bioworld Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Translation initiation factor eIF-2B subunit delta; eIF-2B GDP-GTP exchange factor subunit delta; EIF2B4; EIF2BD

Immunogen: Synthetic peptide, corresponding to Human EIF2B4.

Conjugate: Unconjugated

Modification: Unmodified