EIF2C3/Argonaute 3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90453-50, BS90453-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9H9G7(Human) Q8CJF9(Mouse) Unigene:92025(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, FC
All Applications: WB:1:1,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: EIF2C3/Argonaute 3 polyclonal antibody detects endogenous levels of EIF2C3/Argonaute 3 protein.
Bioworld Molecular Weight: 97 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: Argonaute3, hAgo3, eIF-2C 3, eIF2C 3, AGO3, 5730550L01Rik, Ago 3, Ago3, AGO3, argonaute 3, Argonaute3, eIF 2C 3, eIF-2C 3, eIF2C 3, Eif2c3, EIF2C3 protein, Eukaryotic translation initiation factor 2C 3, Eukaryotic translation initiation factor 2C3, FLJ12765, hAgo3, MGC86946, Protein argonaute-3,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified