EIF2S2 Polyclonal Antibody-BS61711

EIF2S2 Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS61711-50, BS61711-100

Sizes: 50µl, 100µl

Swiss-Prot: P20042

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF2 exists as a heterotrimeric complex of eIF2α, eIF2β and eIF2γ. eIF2 functions in the early stages of protein synthesis, by forming a ternary complex with GTP and tRNA. This complex binds to the 40S ribosomal subunit, followed by mRNA binding to 40S to form the 43S preinitiation complex, the release of eIF2 from 40S, and the hydrolysis of GTP. Phosphorylation of eIF2α correlates with inhibition of translation initiation.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: EIF2S2 polyclonal antibody detects endogenous levels of EIF2S2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EIF2S2 polyclonal antibody at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: CT26 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of EIF2S2 polyclonal antibody in paraffin-embedded human stomach cancer tissue at 1:50.

Bioworld Molecular Weight: ~ 50 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Eukaryotic translation initiation factor 2 subunit 2; Eukaryotic translation initiation factor 2 subunit beta; eIF-2-beta; EIF2S2; EIF2B

Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human EIF2S2.

Conjugate: Unconjugated

Modification: Unmodified