EIF3E Polyclonal Antibody - E-AB-61799

EIF3E Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-61799-60, E-AB-61799-120, E-AB-61799-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: EIF3E

Target Synonym: EIF3E; EIF3-P48; EIF3S6; INT6; eIF3-p46

Research Areas: Cancer,Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P60228

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3 complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. Required for nonsense-mediated mRNA decay (NMD; may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.

Concentration: 1 mg/mL

Immunogen: Recombinant fusion protein of human EIF3E

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 52kDa

Observed MW: 52kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only