Product Description

eIF4B (phospho-S422) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4311-50, BS4311-100

Swiss-Prot: P23588

Host: Rabbit

eIF4B (phospho-S422) Polyclonal Antibody -BS4311

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-eIF4B (S422) polyclonal antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser422

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-eIF4B (S422) pAb at 1:500 dilution
Lane 1: The Brain tissue lysate of Rat (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: A375 whole cell lysate (40ug)
Lane 4: HCT116 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of p-eIF4B (S422) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Alternative Name: Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B

Immunogen: Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.

Modification: Phosphorylation

Conjugate: Unconjugated