Product Description

eIF4B (phospho-S422) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4311-50, BS4311-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.It is phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1.phosphorylation enhances the affinity of EIF4B for the EIF3 complex

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human eIF4B around the phosphorylation site of Serine 422.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-eIF4B (S422) polyclonal antibody detects endogenous levels of eIF4B protein only when phosphorylated at Ser422

Molecular Weight: ~ 80 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P23588

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only