Product Description

EIF4B Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7572-50, BS7572-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23588

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF, IP,

All Applications: WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200

Background: Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EIF4B polyclonal antibody detects endogenous levels of EIF4B protein.

Bioworld Molecular Weight: ~ 80 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of EIF4B polyclonal antibody, Immunohistochemistry (IHC) analysis of EIF4B polyclonal antibody in paraffin-embedded Human Breast Cancer tissue., Immunofluorescence analysis of U2OS cells, using EIF4B polyclonal antibody

Alternative Name: Eukaryotic translation initiation factor 4B; eIF-4B; EIF4B

Immunogen: Recombinant full length Human EIF4B.

Conjugate: Unconjugated

Modification: Unmodified