eIF4E (Phospho-S209) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94012-50, BS94012-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P06730(Human) P63073(Mouse) P63074(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC, IP,
All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: eIF4E (Phospho-S209) polyclonal antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.
Bioworld Molecular Weight: 25 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-eIF4E(S209) on different lysates using anti-Phospho-eIF4E(S209) antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse spleen
Lane 2: Rat spleen, ICC staining Phospho-eIF4E(S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: eIF-4E, eIF4E, EIF4E, AUTS19, CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF 4F, eIF-4E, eIF-4F 25 kDa subunit, eIF4E, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4 E, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E like 1, IF4E, Messanger RNA Cap Binding Protein eIF 4E, MGC111573, mRNA cap binding protein, mRNA cap-binding protein,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser209 of human eIF4E.
Conjugate: Unconjugated
Modification: Phosphorylation