eIF4EBP1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90455-50, BS90455-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q13541(Human) Q60876(Mouse) Q62622(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC, IP, , FC
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: eIF4EBP1 polyclonal antibody detects endogenous levels of eIF4EBP1 protein.
Bioworld Molecular Weight: 17 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of eIF4EBP1 on different lysates using anti-eIF4EBP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: HepG2
Lane 3: 293, ICC staining eIF4EBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: 4E-BP1, eIF4E-binding protein 1, PHAS-I, EIF4EBP1, 4E-BP1, 4EBP1, 4EBP1, BP 1, eIF4E binding protein 1, eIF4E-binding protein 1, Eif4ebp1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, PHASI, Phosphorylated heat- and acid-stable protein regulated by insulin 1,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified