eIF4G (Q1103) Polyclonal Antibody
Catalogue Numbers:BS1534-50, BS1534-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q04637
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: IHC, IF
All Applications: IHC: 1:50~1:200
IF: 1:50~1:200
Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E is a 25 kDa protein that binds to the mRNA “cap” during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: eIF4G (Q1103) polyclonal antibody detects endogenous levels of eIF4G protein.
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Immunohistochemistry (IHC) analyzes of eIF4G (Q1103) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Alternative Name: eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220; EIF4G1; 1981; EIF4G1; 600495; Q04637; DKFZp686A1451; EIF4F; EIF4G; p220;
Immunogen: Synthetic peptide, corresponding to amino acids 1080-1130 of Human eIF4G.
Conjugate: Unconjugated
Modification: Unmodified