Product Description

EIF4G1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS8980-50, BS8980-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q04637

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:50 - 1:200

Background: The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 220KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using EIF4G1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded human colon carcinoma using EIF4G1 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunoprecipitation analysis of 300ug extracts of Hela cells using 3ug EIF4G1 antibody. Western blot was performed from the immunoprecipitate using EIF4G1 antibody at a dilution of 1:500.

Alternative Name: EIF4G1; EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18

Immunogen: A synthetic peptide of human EIF4G1(NP_886553.3).

Conjugate: Unconjugated

Modification: Unmodified