Product Description

EIF5A Polyclonal Antibody

Catalogue Numbers: BS60424-50, BS60424-100

Sizes: 50µl, 100µl

Alternative Name: Eukaryotic translation initiation factor 5A-1; eIF-5A-1; eIF-5A1; Eukaryotic initiation factor 5A isoform 1; eIF-5A; Rev-binding factor; eIF-4D; eIF4D

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P63241

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A, maps to chromosome 17p13-p12 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A's biochemical activity and for cellular proliferative signaling. In addition, eIF5A is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A interaction with Rev leads to a block of the viral replication cycle.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EIF5A polyclonal antibody detects endogenous levels of EIF5A protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of EIF5A polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human EIF5A.

Conjugate: Unconjugated

Modification: Unmodified