eIF5A Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90456-50, BS90456-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P63241(Human) P63242(Mouse) Q3T1J1(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC, IP, FC
All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100
Background: In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A, maps to chromosome 17p13.1 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A's biochemical activity and for cellular proliferative signaling. In addition, eIF5A is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A interaction with Rev leads to a block of the viral replication cycle.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: eIF5A polyclonal antibody detects endogenous levels of eIF5A protein.
Bioworld Molecular Weight: 18 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of eIF5A on different lysates using anti-eIF5A antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: MCF-7, ICC staining eIF5A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: eIF-5A-1, eIF-5A1, eIF-5A, EIF5A, eIF 4D, eIF 5A 1, EIF 5A, eIF 5A1, eIF-4D, eIF-5A, eIF-5A-1, eIF-5A1, eIF4D, eif5a, EIF5A1, eIF5AI, Eukaryotic initiation factor 5A, Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A 1, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, IF5A1, MGC104255, MGC99547, Rev binding factor, Rev-binding factor, uORF A, uORF,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified