Product Description

Elf-4 Polyclonal Antibody

Catalogue Numbers: BS5710-50, BS5710-100

Sizes: 50µl, 100µl

Alternative Name: ETS-related transcription factor Elf-4; E74-like factor 4; Myeloid Elf-1-like factor; ELF4; ELFR; MEF

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99607

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Ets-1 family of transcription factors has a conserved DNA binding domain through which it plays an important role in cellular proliferation, differentiation, tematopoiesis and angiogenesis. This domain, also known as the Ets domain, binds to DNA sequences containing the consensus sequence 5'-WGGA-3', which is known as the Ets-binding domain. Elf-4, also known as myeloid Elf-1-like factor, ELF4 or MEF, is a 663 amino acid member of the Ets-1 family. Localized to the nucleus, Elf-4 is highly expressed in placenta and myeloid leukemia cells, with lower levels of expression lung, heart, thymus, slpeen, colon, ovary and peripheral blood lymphocytes. Functioning primarily to activate the promoters of hematopoietic growth factor genes, such as GM-CSF, IL-3 and IL-8, Elf-4 has also been shown to activate the Perforin 1 promoter in natural killer (NK) cells, suggesting a possible role in tumorigenesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Elf-4 polyclonal antibody detects endogenous levels of Elf-4 protein.

Bioworld Molecular Weight: ~ 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Elf-4 polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 219-264 of Human Elf-4.

Conjugate: Unconjugated

Modification: Unmodified