ELISA Kit for Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH)
Sizes: 24T, 48T, 96T
Catalogue Numbers: AEA843Po-24, AEA843Po-48, AEA843Po-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Antibody detecting kit
Target: Gonadotropin Releasing Hormone
Alternative Names: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1
Target Species: Pig
Uniprot: P49921
Gene ID: 397516
Featured Series Function: Detects protein (antibody)
Specificity: Reactive with Pig GnRH / Gonadotropin Releasing Hormone
Method: Colormetric
Detection principle: Competitive Inhibition
Detection
range: 3.12-200ng/mL
Sensitivity: 1.15ng/mL
Assay Time: 2h, 30min
Sample Size: 50uL
Recommended/Predicted
Sample Types: Serum
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH).
No significant cross-reactivity or interference between Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Gonadotropin Releasing Hormone Antibody (Anti-GnRH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Endocrinology; Reproductive science; Neuro science; Genetic science; Hormone metabolism;
Research Use Only