ELISA Kit for Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG)
Size: 96T
Catalogue Number: UAEA422Hu-96
Citations, Manuals and MSDS Available upon request.
Target: Myelin Associated Glycoprotein (MAG)
Alternative Names: GMA; S-MAG; Siglec-4; SIGLEC4A; Sialic Acid Binding Ig-Like Lectin 4A
Target Species: Human
Detection range: 1.56-100ng/mL
Sensitivity: 0.59ng/mL
Uniprot: P20916
Gene ID: 4099
Featured Series Function: Detects protein (antigen)
Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids
Assay Time: 2h, 30min
Method: Colormetric
Specificity: Reactive with Human MAG / Myelin Associated Glycoprotein
Detection principle: Double-antibody Sandwich
Sample Size: 100uL
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG).
No significant cross-reactivity or interference between Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Myelin Associated Glycoprotein Antibody (Anti-MAG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Infection immunity; Neuroscience; Autoimmunity;
Research Use Only