ELISA Kit for Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2)
Sizes: 24T, 48T, 96T
Catalogue Numbers: SEB558Hu-24, SEB558Hu-48, SEB558Hu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Sandwich kit
Target: Cytochrome P450 Family 21 Subfamily A Member 2
Alternative Names: CYP21B; CPS1; CA21H; CAH1; CYP21; CYP21-B; 21-OHase; Cytochrome P450 21B; Steroid 21-hydroxylase; Cytochrome P450, Family 21, Subfamily A, Polypeptide 2
Target Species: Human
Uniprot: P08686
Gene ID: 1589
Featured Series Function: Detects protein (regular version)
Specificity: Reactive with Human CYP21A2 / Cytochrome P450 Family 21 Subfamily A Member 2
Method: Colormetric
Detection principle: Double-antibody Sandwich
Detection
range: 0.156-10ng/mL
Sensitivity: 0.057ng/mL
Assay Time: 3h
Sample Size: 100uL
Recommended/Predicted
Sample Types: Tissue Homogenates, Cell Lysates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2).
No significant cross-reactivity or interference between Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 Family 21 Subfamily A Member 2 (CYP21A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Enzyme & Kinase; Metabolic pathway; Endocrinology; Hormone metabolism;
Research Use Only