ELISA Kit for Glutathione S Transferase (GST)
Size: 96T
Catalogue Number: USEX158Ge-96
Citations, Manuals and MSDS Available upon request.
Target: Glutathione S Transferase (GST)
Alternative Names: GST-Tag
Target Species: General species
Detection range: 1.56-100ng/mL
Sensitivity: 0.57ng/mL
Uniprot: P08515
Featured Series Function: Detects protein (regular version)
Recommended/Predicted Sample Types: Biological Fluids
Assay Time: 3h
Method: Colormetric
Specificity: Reactive with General species GST / Glutathione S Transferase
Detection principle: Double-antibody Sandwich
Sample Size: 100uL
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase (GST).
No significant cross-reactivity or interference between Glutathione S Transferase (GST) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase (GST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase (GST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GST. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated antibody specific to GST. After TMB substrate solution is added, those wells that contain GST will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Use Only