ELISA Kit for Tachykinin, Precursor 1 (TAC1) - UCEC780Hu

ELISA Kit for Tachykinin, Precursor 1 (TAC1)

Size: 96T

Catalogue Number: UCEC780Hu-96

Citations, Manuals and MSDS Available upon request.

Target: Tachykinin, Precursor 1 (TAC1)

Alternative Names: NK2; NKNA; NKA; TAC2; • PPT; Protachykinin-1; Substance P; Neurokinin A; Neuromedin L; Substance K; Neuropeptide K; Neuropeptide gamma; C-terminal-flanking peptide

Target Species: Human

Detection range: 123.5-10,000pg/mL

Sensitivity: 51.9pg/mL

Uniprot: P20366

Gene ID: 6863

Featured Series Function: Detects small molecule

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Time: 2h

Method: Colormetric

Specificity: Reactive with Human TAC1 / Tachykinin, Precursor 1

Detection principle: Competitive Inhibition

Sample Size: 50uL

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Tachykinin, Precursor 1 (TAC1).

No significant cross-reactivity or interference between Tachykinin, Precursor 1 (TAC1) and analogues was observed.

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.

To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards;

2. Add 50µL standard or sample to each well.

And then add 50µL prepared Detection Reagent A immediately.

Shake and mix. Incubate 1 hour at 37°C;

3. Aspirate and wash 3 times;

4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;

5. Aspirate and wash 5 times;

6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;

7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tachykinin, Precursor 1 (TAC1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tachykinin, Precursor 1 (TAC1) and unlabeled Tachykinin, Precursor 1 (TAC1) (Standards or samples) with the pre-coated antibody specific to Tachykinin, Precursor 1 (TAC1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample.

Research Area: Neuroscience;

Research Use Only