ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)
Sizes: 24T, 48T, 96T
Catalogue Numbers: CEA218Hu-24, CEA218Hu-48, CEA218Hu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Competitive kit
Target: Transforming Growth Factor Beta 2
Alternative Names: TGF-B2; TGF-Beta2; G-TSF; LAP; Cetermin; Polyergin; Latency-associated peptide; BSC-1 cell growth inhibitor; Glioblastoma-derived T-cell suppressor factor
Target Species: Human
Uniprot: P61812
Gene ID: 7042
Featured Series Function: Detects small molecule
Specificity: Reactive with Human TGFb2 / Transforming Growth Factor Beta 2
Method: Colormetric
Detection principle: Competitive Inhibition
Detection
range: 24.69-2,000pg/mL
Sensitivity: 9.03pg/mL
Assay Time: 2h
Sample Size: 50uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 2 (TGFb2).
No significant cross-reactivity or interference between Transforming Growth Factor Beta 2 (TGFb2) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 2 (TGFb2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Transforming Growth Factor Beta 2 (TGFb2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Transforming Growth Factor Beta 2 (TGFb2) and unlabeled Transforming Growth Factor Beta 2 (TGFb2) (Standards or samples) with the pre-coated antibody specific to Transforming Growth Factor Beta 2 (TGFb2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Transforming Growth Factor Beta 2 (TGFb2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Transforming Growth Factor Beta 2 (TGFb2) in the sample.
Research Area: Cytokine; Infection immunity;
Research Use Only