Product Description

Elk-1 (E33) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1948-50, BS1948-100

Sizes: 50µl, 100µl

Swiss-Prot: P19419

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Elk-1 (E33) polyclonal antibody detects endogenous levels of Elk-1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Elk-1 (E33) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: HEK293T whole cell lysate (40ug)
Lane 3: U-87MG whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Elk-1 (E33) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 45 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: ELK 1; Elk1; ELK1 member of ETS oncogene family; ELK1 protein; ELK2 member of ETS oncogene family; ETS domain containing protein Elk 1; ETS domain containing protein Elk1; ETS domain protein Elk1; ETS domain-containing protein Elk-1; ETS like gene 1; Member of ETS oncogene family; Oncogene Elk1; Tyrosine kinase (ELK1) oncogene;

Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Elk-1.

Conjugate: Unconjugated

Modification: Unmodified