Elongin A1 (R129) Polyclonal Antibody-BS2530

Elongin A1 (R129) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS2530-50, BS2530-100

Sizes: 50µl, 100µl

Swiss-Prot: Q14241

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Individuals harboring germline mutations in the tumor suppressor gene von Hippel-Lindau (VHL) exhibit an increased susceptibility to a variety of tumors including renal carcinoma, hemangio-blastoma of the central nervous system and pheochromo-cytoma. The Elongin (SIII) complex has been identified as the functional target of the VHL protein. Elongin (SIII) is a heterotrimer composed of a transcriptional active subunit designated Elongin A and two regulatory subunits designated Elongin B and Elongin C. VHL functions by binding to the Elongin B and C subunits, inhibiting the transcriptional efficacy of the Elongin (SIII) complex. The VHL protein migrates with an apparent molecular weight of 38 kDa. The Elongin A subunit is 773 amino acids in length and has an apparent molecular weight of 110 kDa, while Elongin B and C are 18 kDa and 15 kDa proteins, respectively.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Elongin A1 (R129) polyclonal antibody detects endogenous levels of Elongin A1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Elongin A1 (R129) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: PC3 whole cell lysate (40ug)
Lane 3: K562 whole cell lysate (40ug)
Lane 4: H9C2 whole cell lysate (40ug)
Lane 5: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of Elongin A1 (R129) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~78 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Transcription elongation factor B polypeptide 3; RNA polymerase II transcription factor SIII subunit A1; SIII p110; Elongin A; EloA; Elongin 110 kDa subunit; TCEB3;

Immunogen: Synthetic peptide, corresponding to amino acids 98-142 of Human Elongin A1.

Conjugate: Unconjugated

Modification: Unmodified