Product Description

Elongin A3 (E403) Polyclonal Antibody

Catalogue Numbers: BS2412-50, BS2412-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q8NG57

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The elongin (SIII) complex is composed of a transcriptionally active A subunit and two small regulatory subunits, B and C. This complex stimulates the transcription elongation rate of RNA polyermase II by suppressing time spent at template-encoded arresting sites along DNA. Elongin A3, also known as RNA polymerase II transcription factor SIII subunit A3 and Transcription elongation factor B polypeptide 3C, is a 546 amino acid nuclear protein that functions as a general transcription elongation factor that increases RNA polymerase II transcription elongation and forms a stable complex with Elongin BC. In contrast to Elongin A, transcriptional activity of Elongin A3 is not stimulated by Elongin BC. Elongin A3 contains one TFIIS N-terminal domain and is ubiquitously expressed in human tissue.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Elongin A3 (E403) polyclonal antibody detects endogenous levels of Elongin A3 protein.

Extra Notes: Western blot (WB) analysis of Elongin A3 (E403) polyclonal antibody at 1:500 dilution
Lane1:Jurkat cell lysate
Lane2:Mouse kidney tissue lysate
Lane3:Rat kidney tissue lysate

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: TCEB3C; Elongin-A3; Elongin A3; ElonginA3; EloA3; TCEB3L2

Immunogen: Synthetic peptide, corresponding to amino acids 370-422 of Human Elongin A3.

Conjugate: Unconjugated

Modification: Unmodified