Emi1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90461-50, BS90461-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q9UKT4(Human) Q7TSG3(Mouse)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC IHC
All Applications: ICC:1:50-1:200
IHC:1:50-1:200
WB:1:500
Background: Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: Emi1 polyclonal antibody detects endogenous levels of Emi1 protein.
Bioworld Molecular Weight: 50 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Emi1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Emi1 in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: FBXO5, EMI1, FBX5, Early mitotic inhibitor 1, Emi 1, EMI1, F box only protein 5, F box protein 5, F box protein Fbx 5, F box protein Fbx5, F-box only protein 5, FBX5, FBX5, Fbxo31, fbxo5,
Immunogen: Recombinant protein within human Emi1 aa 50-250.
Conjugate: Unconjugated
Modification: Unmodified