EN2 Polyclonal Antibody-BS77280

EN2 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS77280-50, BS77280-100 Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Swiss-Prot: P19622 Host: Rabbit Reactivity: Human, Mouse, Rat Applications: WB All Applications: WB,1:500 - 1:2000 Background: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008] Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Unmodification Extra Notes: Western blot analysis of extracts of various cell lines, using EN2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. Note: For research use only, not for use in diagnostic procedure. Bioworld Molecular Weight: 33kDa Alternative Name: EN2 Immunogen: A synthetic peptide of human EN2(NP_001418.2). Conjugate: Unconjugated Modification: Unmodified