ENDOG Polyclonal Antibody
Catalogue Numbers: BS60425-50, BS60425-100
Sizes: 50µl, 100µl
Alternative Name: Endonuclease G, mitochondrial; Endo G
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q14249
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria (1). This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA(ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ENDOG polyclonal antibody detects endogenous levels of ENDOG protein.
Bioworld Molecular Weight: ~ 35 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ENDOG polyclonal antibody at 1:500 dilution
Lane 1: HepG2 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human ENDOG.
Conjugate: Unconjugated
Modification: Unmodified