Product Description

EPG5 Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67191-50, BS67191-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9HCE0

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Involved in autophagy. May play a role in a late step of autophagy, such as clearance of autophagosomal cargo. Plays a key role in innate and adaptive immune response triggered by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides from pathogens, and mediated by the nucleotide-sensing receptor TLR9. It is necessary for the translocation of CpG dinucleotides from early endosomes to late endosomes and lysosomes, where TLR9 is located (PubMed:29130391).

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 152% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of EPG5 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of EPG5 expression in Hela (A), HepG2 (B), MCF7 (C), C6 (D) whole cell lysates., Immunohistochemical analysis of EPG5 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 282 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: KIAA1632; Ectopic P granules protein 5 homolog

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EPG5. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified